Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo

Etheridge, Thomas J, Villahermosa, Desiree, Campillo-Funollet, Eduard, Herbert, Alex David, Irmisch, Anja, Watson, Adam T, Dang, Hung Q, Osborne, Mark A, Oliver, Antony W, Carr, Antony M and Murray, Johanne M (2021) Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo. eLife, 10. a68579. ISSN 2050-084X

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Abstract

The essential Smc5/6 complex is required in response to replication stress and is best known for ensuring the fidelity of homologous recombination. Using single-molecule tracking in live fission yeast to investigate Smc5/6 chromatin association, we show that Smc5/6 is chromatin associated in unchallenged cells and this depends on the non-SMC protein Nse6. We define a minimum of two Nse6-dependent sub-pathways, one of which requires the BRCT-domain protein Brc1. Using defined mutants in genes encoding the core Smc5/6 complex subunits we show that the Nse3 double-stranded DNA binding activity and the arginine fingers of the two Smc5/6 ATPase binding sites are critical for chromatin association. Interestingly, disrupting the ssDNA binding activity at the hinge region does not prevent chromatin association but leads to elevated levels of gross chromosomal rearrangements during replication restart. This is consistent with a downstream function for ssDNA binding in regulating homologous recombination.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
SWORD Depositor: Mx Elements Account
Depositing User: Mx Elements Account
Date Deposited: 19 Apr 2021 07:53
Last Modified: 09 Jun 2021 16:31
URI: http://sro.sussex.ac.uk/id/eprint/98475

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