Peptide microarray based analysis of antibody responses to SARS-CoV-2 identifies unique epitopes with potential for diagnostic test development

Holenya, Pavlo, Lange, Paul Joris, Reimer, Ulf, Woltersdorf, Wolfram, Panterodt, Thomas, Glas, Michael, Wasner, Mark, Eckey, Maren, Drosch, Michael, Hollidt, Jörg‐Michael, Naumann, Michael, Kern, Florian, Wenschuh, Holger, Lange, Robert, Schnatbaum, Karsten and Bier, Frank F (2021) Peptide microarray based analysis of antibody responses to SARS-CoV-2 identifies unique epitopes with potential for diagnostic test development. European Journal of Immunology. ISSN 0014-2980

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Abstract

Humoral immunity to the Severe Adult Respiratory Syndrome (SARS) Coronavirus (CoV)‐2 is not fully understood yet but is a crucial factor of immune protection. The possibility of antibody cross‐reactivity between SARS‐CoV‐2 and other human coronaviruses (HCoVs) would have important implications for immune protection but also for the development of specific diagnostic ELISA tests. Using peptide microarrays, n = 24 patient samples and n = 12 control samples were screened for antibodies against the entire SARS‐CoV‐2 proteome as well as the Spike (S), Nucleocapsid (N), VME1 (V), R1ab, and Protein 3a (AP3A) of the HCoV strains SARS, MERS, OC43 and 229E. While widespread cross‐reactivity was revealed across several immunodominant regions of S and N, IgG binding to several SARS‐CoV‐2‐derived peptides provided statistically significant discrimination between COVID‐19 patients and controls. Selected target peptides may serve as capture antigens for future, highly COVID‐19‐specific diagnostic antibody tests.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Clinical and Experimental Medicine
SWORD Depositor: Mx Elements Account
Depositing User: Mx Elements Account
Date Deposited: 30 Mar 2021 13:27
Last Modified: 30 Mar 2021 13:30
URI: http://sro.sussex.ac.uk/id/eprint/98151

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