KOYA_European_Journal_of_Neuroscience_APR_2020_author_copy.pdf (3.29 MB)
Extinction of cue-evoked food seeking recruits a GABAergic interneuron ensemble in the dorsal medial prefrontal cortex of mice
journal contribution
posted on 2023-06-07, 06:55 authored by Leonie S Brebner, Joseph J Ziminski, Gabriella Margetts-Smith, Meike C Sieburg, Catherine HallCatherine Hall, Tristan G Heintz, Leon Lagnado, Johannes Hirrlinger, Hans CrombagHans Crombag, Eisuke KoyaEisuke KoyaAnimals must quickly adapt food-seeking strategies to locate nutrient sources in dynamically changing environments. Learned associations between food and environmental cues that predict its availability promote food-seeking behaviors. However, when such cues cease to predict food availability, animals undergo 'extinction' learning, resulting in the inhibition of food-seeking responses. Repeatedly activated sets of neurons, or 'neuronal ensembles', in the dorsal medial prefrontal cortex (dmPFC) are recruited following appetitive conditioning and undergo physiological adaptations thought to encode cue-reward associations. However, little is known about how the recruitment and intrinsic excitability of such dmPFC ensembles are modulated by extinction learning. Here, we used in vivo 2-Photon imaging in male Fos-GFP mice that express green fluorescent protein (GFP) in recently behaviorally-activated neurons to determine the recruitment of activated pyramidal and GABAergic interneuron mPFC ensembles during extinction. During extinction, we revealed a persistent activation of a subset of interneurons which emerged from a wider population of interneurons activated during the initial extinction session. This activation pattern was not observed in pyramidal cells, and extinction learning did not modulate the excitability properties of activated neurons. Moreover, extinction learning reduced the likelihood of reactivation of pyramidal cells activated during the initial extinction session. Our findings illuminate novel neuronal activation patterns in the dmPFC underlying extinction of food-seeking, and in particular, highlight an important role for interneuron ensembles in this inhibitory form of learning.
History
Publication status
- Published
File Version
- Accepted version
Journal
European Journal of NeuroscienceISSN
1460-9568Publisher
WileyExternal DOI
Event location
FranceDepartment affiliated with
- Psychology Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2020-05-04First Open Access (FOA) Date
2020-05-04First Compliant Deposit (FCD) Date
2020-05-01Usage metrics
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