Schalbetter et al 2019.pdf (4.56 MB)
Principles of meiotic chromosome assembly revealed in S. cerevisiae
journal contribution
posted on 2023-06-09, 20:19 authored by Stephanie A Schalbetter, Geoffrey Fudenberg, Jonathan Baxter, Katherine S Pollard, Matt NealeMatt NealeDuring meiotic prophase, chromosomes organise into a series of chromatin loops emanating from a proteinaceous axis, but the mechanisms of assembly remain unclear. Here we use Saccharomyces cerevisiae to explore how this elaborate three-dimensional chromosome organisation is linked to genomic sequence. As cells enter meiosis, we observe that strong cohesin-dependent grid-like Hi-C interaction patterns emerge, reminiscent of mammalian interphase organisation, but with distinct regulation. Meiotic patterns agree with simulations of loop extrusion with growth limited by barriers, in which a heterogeneous population of expanding loops develop along the chromosome. Importantly, CTCF, the factor that imposes similar features in mammalian interphase, is absent in S. cerevisiae, suggesting alternative mechanisms of barrier formation. While grid-like interactions emerge independently of meiotic chromosome synapsis, synapsis itself generates additional compaction that matures differentially according to telomere proximity and chromosome size. Collectively, our results elucidate fundamental principles of chromosome assembly and demonstrate the essential role of cohesin within this evolutionarily conserved process.
Funding
Spatial regulation of meiotic recombination; G2004; WELLCOME TRUST; 200843/Z/16/Z
Biochemical reconstitution of DNA repair reactions on intact chromatin; G0986; EUROPEAN UNION; 311336
Control and impact of meiotic DNA resection on recombination and genome stability; G1538; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/M010279/1
History
Publication status
- Published
File Version
- Published version
Journal
Nature CommunicationsISSN
2041-1723Publisher
Nature ResearchExternal DOI
Volume
10Article number
a4795Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2020-01-21First Open Access (FOA) Date
2020-01-21First Compliant Deposit (FCD) Date
2020-01-21Usage metrics
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