Proteomic analysis of human placental syncytiotrophoblast microvesicles in preeclampsia.pdf (789.6 kB)
Proteomic analysis of human placental syncytiotrophoblast microvesicles in preeclampsia
journal contribution
posted on 2023-06-09, 19:35 authored by Sonia Baig, Narasimhan Kothandaraman, Jayapal Manikandan, Li Rong, Kim Huey EE, Jeffrey Hill, Chin Wee Lai, Wan Yu Tan, Felicia Yeoh, Anita Kale, Lin Lin Su, Arijit Biswas, Sheila Vasoo, Mahesh ChoolaniBackground Placental syncytiotrophoblast microvesicles (STBM) are shed into the maternal circulation during normal pregnancy. STBM circulate in significantly increased amounts in preeclampsia (PE) and are considered to be among contributors to the exaggerated proinflammatory, procoagulant state of PE. However, protein composition of STBM in normal pregnancy and PE remains unknown. We therefore sought to determine the protein components of STBM and whether STBM protein expressions differ in preeclamptic and normal pregnancies. Patients with PE (n?=?3) and normal pregnant controls (n?=?6) were recruited. STBM were prepared from placental explant culture supernatant. STBM proteins were analyzed by a combination of 1D Gel-LC-MS/MS. Protein expressions levels were quantified using spectral counts and validated by immunohistochemistry. Results Over 400 proteins were identified in the STBM samples. Among these, 25 proteins were found to be differentially expressed in preeclampsia compared to healthy pregnant controls, including integrins, annexins and histones. Conclusion STBM proteins include those that are implicated in immune response, coagulation, oxidative stress, apoptosis as well as lipid metabolism pathways. Differential protein expressions of STBM suggest their pathophysiological relevance in PE.
History
Publication status
- Published
File Version
- Published version
Journal
Clinical ProteomicsISSN
1559-0275Publisher
BMCExternal DOI
Volume
11Page range
1-8Department affiliated with
- Biochemistry Publications
Research groups affiliated with
- Sussex Drug Discovery Centre Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2019-11-08First Open Access (FOA) Date
2019-11-08First Compliant Deposit (FCD) Date
2019-11-08Usage metrics
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