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A sensor kinase controls turgor-driven plant infection by the rice blast fungus
journal contribution
posted on 2023-06-09, 19:34 authored by Lauren S Ryder, Yasin F Dagdas, Michael J Kershaw, Chandrasekhar VenkataramanChandrasekhar Venkataraman, Anotida MadzvamuseAnotida Madzvamuse, Xia Yan, Neftaly Cruz-Mireles, Darren M Soanes, Miriam Oses-Ruiz, Vanessa StylesVanessa Styles, Jan Sklenar, Frank L H Menke, Nicholas J TalbotThe blast fungus Magnaporthe oryzae gains entry to its host plant by means of a specialized pressure-generating infection cell called an appressorium, which physically ruptures the leaf cuticle. Turgor is applied as an enormous invasive force by septin-mediated reorganization of the cytoskeleton and actin-dependent protrusion of a rigid penetration hypha. However, the molecular mechanisms that regulate the generation of turgor pressure during appressorium-mediated infection of plants remain poorly understood. Here we show that a turgor-sensing histidine–aspartate kinase, Sln1, enables the appressorium to sense when a critical turgor threshold has been reached and thereby facilitates host penetration. We found that the Sln1 sensor localizes to the appressorium pore in a pressure-dependent manner, which is consistent with the predictions of a mathematical model for plant infection. A ?sln1 mutant generates excess intracellular appressorium turgor, produces hyper-melanized non-functional appressoria and does not organize the septins and polarity determinants that are required for leaf infection. Sln1 acts in parallel with the protein kinase C cell-integrity pathway as a regulator of cAMP-dependent signalling by protein kinase A. Pkc1 phosphorylates the NADPH oxidase regulator NoxR and, collectively, these signalling pathways modulate appressorium turgor and trigger the generation of invasive force to cause blast disease.
Funding
InCeM: Research Training Network on Integrated Component Cycling in Epithelial Cell Motility; G1546; EUROPEAN UNION
New predictive mathematical and computational models in experimental sciences; G1949; ROYAL SOCIETY; WM160017
Unravelling new mathematics for 3D cell migration; G1438; LEVERHULME TRUST; RPG-2014-149
History
Publication status
- Published
File Version
- Accepted version
Journal
NatureISSN
0028-0836Publisher
Nature ResearchExternal DOI
Volume
574Page range
423-427Department affiliated with
- Mathematics Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2019-11-07First Open Access (FOA) Date
2020-04-10First Compliant Deposit (FCD) Date
2019-11-06Usage metrics
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