Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system

Okawa, Haruhisa, Yu, Wan-Qing, Matti, Ulf, Scwarz, Karin, Odermatt, Benjamin, Zhong, Haining, Tsukamoto, Yoshihiko, Lagnado, Leon, RIeke, Fred, Schmitz, Frank and Wong, Rachel O L Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system. Nature Communications, 10 (2167). pp. 1-13. ISSN 2041-1723

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Abstract

Ribbon synapses transmit information in sensory systems, but their development is not well understood. To test the hypothesis that ribbon assembly stabilizes nascent synapses, we performed simultaneous time-lapse imaging of fluorescently-tagged ribbons in retinal cone bipolar cells (BCs) and postsynaptic densities (PSD95-FP) of retinal ganglion cells (RGCs). Ribbons and PSD95-FP clusters were more stable when these components colocalized at synapses. However, synapse density on ON-alpha RGCs was unchanged in mice lacking ribbons (ribeye knockout). Wildtype BCs make both ribbon-containing and ribbon-free synapses with these GCs even at maturity. Ribbon assembly and cone BC-RGC synapse maintenance are thus regulated independently. Despite the absence of synaptic ribbons, RGCs continued to respond robustly to light stimuli, although quantitative examination of the responses revealed reduced frequency and contrast sensitivity.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Research Centres and Groups: Sussex Neuroscience
Depositing User: Leon Lagnado
Date Deposited: 03 Apr 2019 13:37
Last Modified: 01 Jul 2019 13:01
URI: http://sro.sussex.ac.uk/id/eprint/82969

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Project NameSussex Project NumberFunderFunder Ref
Synaptic computation in the visual systemG1321WELLCOME TRUST102905/Z/13/Z