Zhang et al 2018 Subcellular peptides Sci Rep.pdf (4.66 MB)
Subcellular peptide localization in single identified neurons by capillary microsampling mass spectrometry
journal contribution
posted on 2023-06-09, 14:34 authored by Linwen Zhang, Nikkita Khattar, Ildiko KemenesIldiko Kemenes, George KemenesGeorge Kemenes, Zita Zrinyi, Zsolt Pirger, Akos VertesSingle cell mass spectrometry (MS) is uniquely positioned for the sequencing and identification of peptides in rare cells. Small peptides can take on different roles in subcellular compartments. Whereas some peptides serve as neurotransmitters in the cytoplasm, they can also function as transcription factors in the nucleus. Thus, there is a need to analyze the subcellular peptide compositions in identified single cells. Here, we apply capillary microsampling MS with ion mobility separation for the sequencing of peptides in single neurons of the mollusk Lymnaea stagnalis, and the analysis of peptide distributions between the cytoplasm and nucleus of identified single neurons that are known to express cardioactive Phe-Met-Arg-Phe amide-like (FMRFamide-like) neuropeptides. Nuclei and cytoplasm of Type 1 and Type 2?F group (Fgp) neurons were analyzed for neuropeptides cleaved from the protein precursors encoded by alternative splicing products of the FMRFamide gene. Relative abundances of nine neuropeptides were determined in the cytoplasm. The nuclei contained six of these peptides at different abundances. Enabled by its relative enrichment in Fgp neurons, a new 28-residue neuropeptide was sequenced by tandem MS.
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- Published
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- Published version
Journal
Scientific ReportsISSN
2045-2322Publisher
Springer NatureExternal DOI
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8Page range
1227Department affiliated with
- Neuroscience Publications
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- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2018-08-23First Open Access (FOA) Date
2018-08-23First Compliant Deposit (FCD) Date
2018-08-21Usage metrics
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