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Demonstration of viral antibodies by pseudotype virus neutralization

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posted on 2023-06-09, 14:32 authored by Edward WrightEdward Wright
Serological studies of rabies viruses (RABV) using pseudotyped viruses (PV) can be undertaken in low-containment laboratories, are highly sensitive and specific, and highly adaptable, so can be tailored to the individual requirements of each laboratory. These facets stem from the fact that PV are replication incompetent, recombinant viruses that can be engineered to contain one of several different reporter genes and a range of different RABV glycoproteins (G) protruding from their viral membrane. Their stability when stored at a wide range of temperatures and through several rounds of freeze-thawing, coupled with the minimal loss of viral titer following lyophilization, further highlights their suitability as reagents for use in resource-limited countries.

History

Publication status

  • Published

Publisher

Elsevier

Page range

147-156

Book title

Current laboratory techniques in rabies diagnosis, research and prevention

ISBN

9780128000144

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Editors

Thirumeni Nagarajan, Charles Rupprecht

Legacy Posted Date

2018-08-14

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