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Schizosaccharomyces pombe MutSa and MutLa maintain stability of tetra-nucleotide repeats and Msh3 of hepta-nucleotide repeats

journal contribution
posted on 2023-06-09, 11:55 authored by Desiree Villahermosa CaballeroDesiree Villahermosa Caballero, Olaf Christensen, Karen Knapp, Oliver Fleck
Defective mismatch repair (MMR) in humans is associated with colon cancer and instability of microsatellites, that is, DNA sequences with one or several nucleotides repeated. Key factors of eukaryotic MMR are the heterodimers MutSa (Msh2-Msh6), which recognizes base-base mismatches and unpaired nucleotides in DNA, and MutLa (Mlh1-Pms1), which facilitates downstream steps. In addition, MutSb (Msh2- Msh3) recognizes DNA loops of various sizes, although our previous data and the data presented here suggest that Msh3 of Schizosaccharomyces pombe does not play a role in MMR. To test microsatellite stability in S. pombe and hence DNA loop repair, we have inserted tetra-, penta-, and hepta-nucleotide repeats in the ade6 gene and determined their Ade+ reversion rates and spectra in wild type and various mutants. Our data indicate that loops with four unpaired nucleotides in the nascent and the template strand are the upper limit of MutSa- and MutLa-mediated MMR in S. pombe. Stability of hepta-nucleotide repeats requires Msh3 and Exo1 in MMR-independent processes as well as the DNA repair proteins Rad50, Rad51, and Rad2FEN1. Most strikingly, mutation rates in the double mutants msh3 exo1 and msh3 rad51 were decreased when compared to respective single mutants, indicating that Msh3 prevents error prone processes carried out by Exo1 and Rad51. We conclude that Msh3 has no obvious function in MMR in S. pombe, but contributes to DNA repeat stability in MMR-independent processes.

History

Publication status

  • Published

File Version

  • Published version

Journal

G3

ISSN

2160-1836

Publisher

Genetics Society of America

Issue

5

Volume

7

Page range

1463-1473

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2018-02-06

First Compliant Deposit (FCD) Date

2018-02-06

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