3837.full.pdf (4.08 MB)
Ephrin A/EphA controls the rostral turning polarity of a lateral commissural tract in chick hindbrain
journal contribution
posted on 2023-06-09, 07:07 authored by Yan Zhu, Sarah GuthrieSarah Guthrie, Fujio MurakamiMost post-crossing commissural axons turn into longitudinal paths to make synaptic connections with their targets. Mechanisms that control their rostrocaudal turning polarity are still poorly understood. We used the hindbrain as a model system to investigate the rostral turning of a laterally located commissural tract, identified as the caudal group of contralateral cerebellar-projecting second-order vestibular neurons (cC-VC). We found that the caudal hindbrain possessed a graded non-permissive/repulsive activity for growing cC-VC axons. This non-permissiveness/repulsion was in part mediated by glycosyl-phosphatidylinositol (GPI)-anchored ephrin A. We further demonstrated that ephrin A2 was distributed in a caudal-high/rostral-low gradient in the caudolateral hindbrain and cC-VC axons expressed EphA receptors. Finally, perturbing ephrin A/EphA signalling both in vitro and in vivo led to rostrocaudal pathfinding errors of post-crossing cC-VC axons. These results suggest that ephrin A/EphA interactions play a key role in regulating the polarity of post-crossing cC-VC axons as they turn into the longitudinal axis.
History
Publication status
- Published
File Version
- Published version
Journal
DevelopmentISSN
0950-1991Publisher
Company of BiologistsExternal DOI
Issue
19Volume
133Page range
3837-3846Department affiliated with
- Neuroscience Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2017-07-11First Open Access (FOA) Date
2017-07-11First Compliant Deposit (FCD) Date
2017-07-11Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC