New technologies for examining neuronal ensembles in drug addiction and fear

Cruz, Fabio C, Koya, Eisuke, Guez-Barber, Danielle H, Bossert, Jennifer M, Lupica, Carl R, Shaham, Yavin and Hope, Bruce T (2013) New technologies for examining neuronal ensembles in drug addiction and fear. Nature reviews: Neuroscience, 14. pp. 743-754. ISSN 1471-003X

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Correlational data suggest that learned associations are encoded within neuronal ensembles. However, it has been difficult to prove that neuronal ensembles mediate learned behaviours because traditional pharmacological and lesion methods, and even newer cell type-specific methods, affect both activated and non-activated neurons. Additionally, previous studies on synaptic and molecular alterations induced by learning did not distinguish between behaviourally activated and non-activated neurons. Here, we describe three new approaches—Daun02 inactivation, FACS sorting of activated neurons and c-fos-GFP transgenic rats — that have been used to selectively target and study activated neuronal ensembles in models of conditioned drug effects and relapse. We also describe two new tools — c-fos-tTA mice and inactivation of CREB-overexpressing neurons — that have been used to study the role of neuronal ensembles in conditioned fear.

Item Type: Article
Schools and Departments: School of Psychology > Psychology
Depositing User: Ellena Adams
Date Deposited: 27 Jan 2017 14:51
Last Modified: 02 Jul 2019 23:55

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