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Identification of a mosquitocidal toxin from Bacillus thuringiensis using mass spectrometry

journal contribution
posted on 2023-06-08, 20:00 authored by Wenfei Zhang, Jie Zhang, Neil CrickmoreNeil Crickmore, Zhongqi Wu, Yiran Yang, Jiangzhao Qian, Hongping Wu, Ruiping Wang, Xuanjun Fang
The Bacillus thuringiensis strain S2160-1 has previously been identified as being highly toxic to mosquito larvae and a viable alternative to strains currently used commercially to control these insects. A PCR approach had identified the presence of four putative insecticidal toxin genes (cry30Ea, cry30 Ga, cry50Ba and cry54Ba) in this strain, but did not identify the genes that encoding three of the main crystal toxin proteins of size 140 and 130 and 30 kDa. In this study we used mass spectrometry to identify the 130 kDa toxin as a rare Cry4 toxin (Cry4Cb3). The gene encoding this toxin was cloned and expressed and the toxin shown to have mosquitocidal activity against Culex quinquefasciatus.

History

Publication status

  • Published

Journal

World Journal of Microbiology and Biotechnology

ISSN

0959-3993

Publisher

Springer Verlag

Issue

12

Volume

30

Page range

3273-3277

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2015-02-12

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