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Fast bi-exponential fluorescence lifetime imaging analysis methods

journal contribution
posted on 2023-06-08, 19:12 authored by David Day-Uei Li, Hongqi Yu, Yu Chen
A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are non-iterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They can be applicable to single-channel or 2D multi-channel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed non-fitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Optics Letters

ISSN

0146-9592

Publisher

Optical Society of America

Issue

3

Volume

40

Page range

336-339

Department affiliated with

  • Engineering and Design Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2014-12-03

First Compliant Deposit (FCD) Date

2014-12-02

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