zbc25241.pdf (2.3 MB)
Oleoyl-coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria
journal contribution
posted on 2023-06-08, 18:00 authored by Obolbek Turapov, Simon WaddellSimon Waddell, Bernard Burke, Sarah Glenn, Asel A Sarybaeva, Griselda Tudo, Gilles Labesse, Danielle I Young, Michael Young, Peter W Andrew, Philip D Butcher, Martin Cohen-Gonsaud, Galina V MukamolovaRegulator of Antimicrobial-Assisted Survival), encoded by Rv1219c in Mycobacterium tuberculosis and bcg_1279c in Mycobacterium bovis BCG, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and to lesser extent palmitoyl-CoA) modulate RaaS binding to DNA and the expression of the genes downstream that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influenced RaaS-mediated mycobacterial improvement of survival and the expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used eliminate non-growing mycobacteria.
History
Publication status
- Published
File Version
- Published version
Journal
Journal of biological chemistryISSN
1083-351XPublisher
American Society for Biochemistry and Molecular Biology, Inc.External DOI
Issue
36Volume
289Page range
25241-25249Department affiliated with
- Global Health and Infection Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2014-08-05First Open Access (FOA) Date
2017-01-10First Compliant Deposit (FCD) Date
2014-08-05Usage metrics
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