J_Cell_Biol-2006-MachÃn-893-903.pdf (3.9 MB)
Transcription of ribosomal genes can cause nondisjunction
journal contribution
posted on 2023-06-08, 17:41 authored by Felix Machin, Jordi Torres-Rosell, Giacomo De Piccoli, Jesus Carballo, Rita Cha, Adam Jarmuz, Luis AragonMitotic disjunction of the repetitive ribosomal DNA (rDNA) involves specialized segregation mechanisms dependent on the conserved phosphatase Cdc14. The reason behind this requirement is unknown. We show that rDNA segregation requires Cdc14 partly because of its physical length but most importantly because a fraction of ribosomal RNA (rRNA) genes are transcribed at very high rates. We show that cells cannot segregate rDNA without Cdc14 unless they undergo genetic rearrangements that reduce rDNA copy number. We then demonstrate that cells with normal length rDNA arrays can segregate rDNA in the absence of Cdc14 as long as rRNA genes are not transcribed. In addition, our study uncovers an unexpected role for the replication barrier protein Fob1 in rDNA segregation that is independent of Cdc14. These findings demonstrate that highly transcribed loci can cause chromosome nondisjunction.
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Publication status
- Published
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- Published version
Journal
The Journal of Cell Biology (JCB)ISSN
0021-9525Publisher
Rockefeller University PressPublisher URL
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Issue
6Volume
173Page range
893-903Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2014-07-11First Open Access (FOA) Date
2014-07-11First Compliant Deposit (FCD) Date
2014-07-11Usage metrics
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