University of Sussex
Browse

File(s) not publicly available

Binding of dynein intermediate chain 2 to paxillin controls focal adhesion dynamics and migration

journal contribution
posted on 2023-06-08, 14:04 authored by Carine Rosse, Katrina Boeckeler, Mark Linch, Simone Radtke, David Frith, Karin Barnouin, Ali Sayed Morsi, Majid HafezparastMajid Hafezparast, Michael Howell, Peter J Parker
In migrating NRK cells, aPKCs control the dynamics of turnover of paxillin-containing focal adhesions (FA) determining migration rate. Using a proteomic approach (two-dimensional fluorescence difference gel electrophoresis), dynein intermediate chain 2 (dynein IC2) was identified as a protein that is phosphorylated inducibly during cell migration in a PKC-regulated manner. By gene silencing and co-immunoprecipitation studies, we show that dynein IC2 regulates the speed of cell migration through its interaction with paxillin. This interaction is controlled by serine 84 phosphorylation, which lies on the aPKC pathway. The evidence presented thus links aPKC control of migration to the dynein control of FA turnover through paxillin

History

Publication status

  • Published

Journal

Journal of Cell Science

ISSN

0021-9533

Publisher

Company of Biologists

Issue

16

Volume

125

Page range

3733-3738

Department affiliated with

  • Neuroscience Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-12-05

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC