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Salt tolerance in Suaeda maritima (L.) Dum: A comparison of mitochondria isolated from green tissues of Suaeda and Pisum
journal contribution
posted on 2023-06-08, 11:30 authored by Tim FlowersMitochondria were isolated from green leaves and stems of the glycophyte Pisum sativum and the halophyte Suaeda maritima. The preparations oxidized malate, succinate, and 2-oxoglutarate as well as externally added NADH. Acceptor control ratios were about 2.8 for mitochondria from Pisum and 1.8 for mitochondria from Suaeda oxidizing malate+pyruvate in 125 mM sodium chloride. The mitochondrial fraction was contaminated with chloroplast fragments which resulted in relatively low rates of oxygen uptake when these were expressed on a protein basis. The addition of sodium chloride at concentrations greater than 200 mM considerably reduced the rates of oxygen uptake by both species in the presence and absence of phosphate acceptor (ADP). Acceptor control ratios were reduced and there was a marked decline in the ADP/O ratio. Sucrose at equivalent molar concentrations had a much less drastic effect on the mitochondria. There was no significant difference in the effects of these two solutes on mitochondria from the two species and the similarity of response is discussed in relation to the cytoplasmic ion content of the halophyte.
History
Publication status
- Published
Journal
Journal of Experimental BotanyISSN
0022-0957Publisher
Oxford University PressExternal DOI
Issue
1Volume
25Page range
101-110Department affiliated with
- Evolution, Behaviour and Environment Publications
Notes
Times Cited: 11 Flowers, tjFull text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-05-21Usage metrics
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