University of Sussex
Browse
Brown_et_al.pdf (147.56 kB)

Ubiquitination and deubiquitination of PCNA in response to stalling of the replication fork

Download (147.56 kB)
journal contribution
posted on 2023-06-08, 07:59 authored by Stephanie Brown, Atsuko Niimi, Alan LehmannAlan Lehmann
Following exposure of human cells to DNA damaging agents that block the progress of the replication fork, mono-ubiquitination of PCNA mediates the switch from replicative DNA polymerases to polymerases specialised for translesion synthesis. We have shown that this modification of PCNA is necessary for the survival of cells after UV-irradiation and methyl methanesulfonate, that it is independent of cell cycle checkpoint activation, and that it persists after UV damage has been removed. In this Extra-view, we compare the regulation and biological significance of PCNA ubiquitination following treatments with UV light and the replication inhibitor hydroxyurea. We show that ubiquitination persists after removal of the replication block in both cases. With UV however, the persistence of ubiquitinated PCNA correlates with disappearance of the PCNA deubiquitinating enzyme USP1, whereas this is not the case for HU. Prevention of PCNA ubiquitination sensitises the cells to killing by both UV and HU.

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Cell Cycle

ISSN

1538-4101

Publisher

Landes Bioscience

Issue

5

Volume

8

Page range

689-692

Pages

4.0

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

First Open Access (FOA) Date

2018-03-22

First Compliant Deposit (FCD) Date

2018-03-22

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC