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Mutation E46K increases phospholipid binding and assembly into filaments of human alpha-synuclein .
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posted on 2023-06-08, 07:36 authored by Woong Choi, Shahin Zibaee, Ross Jakes, Louise SerpellLouise Serpell, Bazbek Davletov, R Anthony Crowther, Michel GoedertMissense mutations (A30P and A53T) in a-synuclein and the overproduction of the wild-type protein cause familial forms of Parkinson's disease and dementia with Lewy bodies. a-Synuclein is the major component of the filamentous Lewy bodies and Lewy neurites that define these diseases at a neuropathological level. Recently, a third missense mutation (E46K) in a-synuclein was described in an inherited form of dementia with Lewy bodies. Here, we have investigated the functional effects of this novel mutation on phospholipid binding and filament assembly of a-synuclein. When compared to the wild-type protein, the E46K mutation caused a significantly increased ability of a-synuclein to bind to negatively charged liposomes, unlike the previously described mutations. The E46K mutation increased the rate of filament assembly to the same extent as the A53T mutation. Filaments formed from E46K a-synuclein often had a twisted morphology with a cross-over spacing of 43 nm. The observed effects on lipid binding and filament assembly may explain the pathogenic nature of the E46K mutation in a-synuclein.
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Publication status
- Published
Journal
FEBS LettersISSN
0014-5793External DOI
Issue
3Volume
576Page range
363-368Department affiliated with
- Biochemistry Publications
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- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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