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Determination of downstream targets of FGF signalling using gene trap and cDNA subtractive approaches
journal contribution
posted on 2023-06-08, 07:33 authored by Hilda Tateossian, Nicola Powles, Robin Dickinson, Michael Ficker, Mark MaconochieSignalling through the fibroblast growth factor family (FGF) of ligands is essential for normal mammalian embryonic development. At a cellular level, many details of the molecular basis of the signal transduction process have been uncovered, but our knowledge of the identity of the downstream effectors of the FGF signal in the developing embryo remains limited. We have used two independent approaches to begin to identify downstream targets of FGF signalling in the embryo: (1) a gene trap approach and (2) cDNA subtraction, using mouse embryonic stem (ES) cells as a cellular system representative of an early window on the developing embryo. Both approaches led to the identification of a number of targets of FGF signalling, and we provide data to show that the chaperone Mrj, the tumour antigen Tum, collapsin mediator response protein Crmp, a novel transcriptional repressor Nac1 and ribophorin are all differentially regulated following FGF signalling. Independent gene trapping of Mrj previously indicated a role for the gene in embryogenesis [Development 126 (1999) 1247], and we present transcript data implicating a number of the newly isolated FGF target genes in different embryonic processes.
History
Publication status
- Published
Journal
Experimental Cell ResearchISSN
0014-4827External DOI
Issue
1Volume
292Page range
101-114Pages
14.0Department affiliated with
- Neuroscience Publications
Notes
I am corresponding author, on work exclusively coming from my labFull text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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