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Interaction of Human DNA Polymerase ? with Monoubiquitinated PCNA: A Possible Mechanism for the Polymerase Switch in Response to DNA Damage
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posted on 2023-06-08, 07:27 authored by Patricia L Kannouche, Jonathan Wing, Alan LehmannAlan LehmannMost types of DNA damage block replication fork progression during DNA synthesis because replicative DNA polymerases are unable to accommodate altered DNA bases in their active sites. To overcome this block, eukaryotic cells employ specialized translesion synthesis (TLS) polymerases, which can insert nucleotides opposite damaged bases. In particular, TLS by DNA polymerase ¿ (pol¿) is the major pathway for bypassing UV photoproducts. How the cell switches from replicative to TLS polymerase at the site of blocked forks is unknown. We show that, in human cells, PCNA becomes monoubiquitinated following UV irradiation of the cells and that this is dependent on the hRad18 protein. Monoubiquitinated PCNA but not unmodified PCNA specifically interacts with pol¿, and we have identified two motifs in pol¿ that are involved in this interaction. Our findings provide an attractive mechanism by which monoubiquitination of PCNA might mediate the polymerase switch.
History
Publication status
- Published
Journal
Molecular CellISSN
1097-2765Publisher
ElsevierExternal DOI
Issue
4Volume
14Page range
491-500Pages
10.0Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Notes
All done in my lab. Paper provided insight into mechanism of switching from replicative to bypass polymerase at sites of DNA damage in human cells.Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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