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Hair cells require phosphatidylinositol 4,5-bisphosphate for mechanical transduction and adaptation

journal contribution
posted on 2023-06-08, 05:22 authored by Moritoshi Hirono, Charlotte S Denis, Guy Richardson, Peter G Gillespie
After opening in response to mechanical stimuli, hair cell transduction channels adapt with fast and slow mechanisms that each depend on Ca2+. We demonstrate here that transduction and adaptation require phosphatidylinositol 4,5-bisphosphate (PIP2) for normal kinetics. PIP2 has a striking distribution in hair cells, being excluded from the basal region of hair bundles and apical surfaces of frog saccular hair cells. Localization of a phosphatidylinositol lipid phosphatase, Ptprq, to these PIP2-free domains suggests that Ptprq maintains low PIP2 levels there. Depletion of PIP2 by inhibition of phosphatidylinositol 4-kinase or sequestration by aminoglycosides reduces the rates of fast and slow adaptation. PIP2 and other anionic phospholipids bind directly to the IQ domains of myosin-1c, the motor that mediates slow adaptation, permitting a strong interaction with membranes and likely regulating the motor's activity. PIP2 depletion also causes a loss in transduction current. PIP2 therefore plays an essential role in hair cell adaptation and transduction.

History

Publication status

  • Published

Journal

Neuron

ISSN

0896-6273

Issue

2

Volume

44

Page range

309-320

Pages

12.0

Department affiliated with

  • Neuroscience Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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