A modified family-B archaeal DNA polymerase with reverse transcriptase activity

Jozwiakowski, Stanislaw and Connolly, Bernard A (2011) A modified family-B archaeal DNA polymerase with reverse transcriptase activity. ChemBioChem, 12 (1). pp. 35-7. ISSN 1439-4227

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Abstract

A hybrid capable of copying stretches of RNA template ∼100 bases long is the first example of a thermostable B family polymerase/reverse transcriptase. The high-fidelity archaeal polymerase contains a fingers domain derived from the more error prone polymerase ζ. For maximum RT activity, fusion with the processivity factor Sso7d is required and the proof-reading exonuclease must be disabled.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
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Depositing User: Stanislaw Jozwiakowski
Date Deposited: 06 Feb 2012 20:06
Last Modified: 25 May 2021 08:29
URI: http://sro.sussex.ac.uk/id/eprint/24109
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