File(s) not publicly available
Identification and characterisation of cultivar-specific 22-kDa heat shock proteins from mitochondria of Pisum sativum
journal contribution
posted on 2023-06-08, 00:43 authored by Carlton K Wood, James R Pratt, Anthony MoorePea plants (Pisum sativum L. cv. Feltham First) exposed to a heat stress of 37 degrees C for 6 h accumulated two low molecular weight (LMW) heat shock proteins (HSPs) of molecular mass 22 kDa. The two LMW HSPs were associated with purified mitochondria. N-terminal amino acid sequencing analysis indicates that the more basic of these proteins is a novel protein. The response of other cultivars of P. sativum to heat shock revealed that up to three 22-kDa HSPs were expressed in a cultivar-specific manner. Evidence presented suggests that the different 22-kDa HSPs arise as a result of there bring multiple 22-kDa HSP genes. The expression of the most basic novel HSP was studied in the Feltham First cultivar using two dimensional SDS-PAGE. Treatment of intact plants with chloramphenicol and cycloheximide prior to heat stress treatment indicated that the LMW HSPs were nuclear encoded and de novo synthesised.
History
Publication status
- Published
Journal
Physiologia PlantarumISSN
0031-9317Publisher
Wiley-BlackwellIssue
3Volume
103Page range
369-376ISBN
0031-9317Department affiliated with
- Biology and Environmental Science Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC