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Expression and crystallization of an N-terminally activated form of the Bacillus thuringiensis Cry1Ca toxin.
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posted on 2023-06-07, 23:08 authored by Thaleia Kouskoura, Candy Tickner, Neil CrickmoreNeil CrickmoreWhen the active form of the Bacillus thuringiensis delta-endotoxin Cry1Ca was expressed in E. coli severe growth retardation was observed. The absence of a short peptide from the N-terminus of the protoxin was responsible for this effect. The introduction of a mutation at an amino acid previously reported as being involved in the initial stages of pore formation within the natural insect target partially abolished the growth retardation effect. We suggest that removal of the N-terminal peptide is a necessary step in toxin activation, the presence of this peptide preventing proper interaction of the toxin with the target membrane. Expression of the truncated toxin in Bacillus thuringiensis also prevented the formation of Cry1Ca crystals.
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Publication status
- Published
Journal
Current MicrobiologyISSN
0343-8651External DOI
Issue
5Volume
43Page range
371-373Pages
3.0Department affiliated with
- Biochemistry Publications
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- No
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- Yes
Legacy Posted Date
2012-02-06Usage metrics
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