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DNA polymerase V-dependent mutator activity in an SOS-induced Escherichia coli strain with a temperature-sensitive DNA polymerase III

journal contribution
posted on 2023-06-07, 13:55 authored by A. R. Timms, Bryn Arthur Bridges
The temperature-sensitive DNA polymerase III (Pol III) encoded by the dnaE486 allele confers a spontaneous mutator activity in SOS-induced bacteria that is largely dependent upon DNA polymerase V (Pol V), encoded by umuD, C. This mutator activity is influenced by the defective proof-reading sub-unit of Pol III encoded by the dnaQ905 (mutD5) allele arguing that Pol V is most likely fixing mutations arising from mismatched primer termini produced by Pol III(486). The size of the dnaQ effect is, however, modest leaving open the possibility that Pol V may be responsible for some of the mutator effect by engaging in bursts of processive activity.

History

Publication status

  • Published

Journal

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

ISSN

0027-5107

Publisher

Elsevier

Volume

499

Page range

97-101

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2007-04-02

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