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DNA polymerase V-dependent mutator activity in an SOS-induced Escherichia coli strain with a temperature-sensitive DNA polymerase III
journal contribution
posted on 2023-06-07, 13:55 authored by A. R. Timms, Bryn Arthur BridgesThe temperature-sensitive DNA polymerase III (Pol III) encoded by the dnaE486 allele confers a spontaneous mutator activity in SOS-induced bacteria that is largely dependent upon DNA polymerase V (Pol V), encoded by umuD, C. This mutator activity is influenced by the defective proof-reading sub-unit of Pol III encoded by the dnaQ905 (mutD5) allele arguing that Pol V is most likely fixing mutations arising from mismatched primer termini produced by Pol III(486). The size of the dnaQ effect is, however, modest leaving open the possibility that Pol V may be responsible for some of the mutator effect by engaging in bursts of processive activity.
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Publication status
- Published
Journal
Mutation Research - Fundamental and Molecular Mechanisms of MutagenesisISSN
0027-5107Publisher
ElsevierExternal DOI
Volume
499Page range
97-101Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2007-04-02Usage metrics
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