ijms-22-06106-v2.pdf (1.17 MB)
A cis-acting mutation in the PxABCG1 promoter is associated with Cry1Ac resistance in Plutella xylostella (L.)
journal contribution
posted on 2023-06-10, 00:09 authored by Jianying Qin, Fan Ye, Linzheng Xu, Xuguo Zhou, Neil CrickmoreNeil Crickmore, Xiaomao Zhou, Youjun Zhang, Zhaojiang GuoThe molecular mechanisms of insect resistance to Cry toxins generated from the bacterium Bacillus thuringiensis (Bt) urgently need to be elucidated to enable the improvement and sustainability of Bt-based products. Although downregulation of the expression of midgut receptor genes is a pivotal mechanism of insect resistance to Bt Cry toxins, the underlying transcriptional regulation of these genes remains elusive. Herein, we unraveled the regulatory mechanism of the downregulation of the ABC transporter gene PxABCG1 (also called Pxwhite), a functional midgut receptor of the Bt Cry1Ac toxin in Plutella xylostella. The PxABCG1 promoters of Cry1Ac-susceptible and Cry1Ac-resistant strains were cloned and analyzed, and they showed clear differences in activity. Subsequently, a dual-luciferase reporter assay, a yeast one-hybrid (Y1H) assay, and RNA interference (RNAi) experiments demonstrated that a cis-mutation in a binding site of the Hox transcription factor Antennapedia (Antp) decreased the promoter activity of the resistant strain and eliminated the binding and regulation of Antp, thereby enhancing the resistance of P. xylostella to the Cry1Ac toxin. These results advance our knowledge of the roles of cis- and trans-regulatory variations in the regulation of midgut Cry receptor genes and the evolution of Bt resistance, contributing to a more complete understanding of the Bt resistance mechanism.
History
Publication status
- Published
File Version
- Published version
Journal
International Journal of Molecular SciencesISSN
1661-6596Publisher
MDPIExternal DOI
Issue
11Volume
22Page range
1-14Article number
a6106Department affiliated with
- Biochemistry Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2021-06-22First Open Access (FOA) Date
2021-06-22First Compliant Deposit (FCD) Date
2021-06-21Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC