BAILY ELSON PIENAAR_CHAPTER.pdf (584.88 kB)
Single-cell approaches for studying the role of mitochondrial DNA in neurodegenerative disease
journal contribution
posted on 2023-06-10, 00:02 authored by Laura BaileyLaura Bailey, Joanna L Elson, Ilse PienaarIn light of accumulating evidence suggestive of cell type-specific vulnerabilities as a result of normal aging processes that adversely affect the brain, as well as age-related neurodegenerative disorders such as Parkinson's disease (PD), the current chapter highlights how we study mitochondrial DNA (mtDNA) changes at a single-cell level. In particular, we comment on increasing questioning of the narrow neurocentric view of such pathologies, where microglia and astrocytes have traditionally been considered bystanders rather than players in related pathological processes. Here we review the contribution made by single-cell mtDNA alterations towards neuronal vulnerability seen in neurodegenerative disorders, focusing on PD as a prominent example. In addition, we give an overview of methodologies that support such experimental investigations. In considering the significant advances that have been made in recent times for developing mitochondria-specific therapies, investigations to account for cell type-specific mitochondrial patterns and how these are altered by disease hold promise for delivering more effective disease-modifying therapeutics.
History
Publication status
- Published
File Version
- Accepted version
Journal
Methods in Molecular BiologyISSN
1064-3745Publisher
HumanaExternal DOI
Volume
2277Page range
299-329Department affiliated with
- Neuroscience Publications
Research groups affiliated with
- Sussex Neuroscience Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2021-06-07First Open Access (FOA) Date
2022-06-04First Compliant Deposit (FCD) Date
2021-06-07Usage metrics
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