AHNAK interacts with the DNA ligase IV-XRCC4 complex and stimulates DNA ligase IV mediated double stranded ligation

Stiff, Thomas, Shtivelman, E., Jeggo, Penny and Kysela, B. (2004) AHNAK interacts with the DNA ligase IV-XRCC4 complex and stimulates DNA ligase IV mediated double stranded ligation. DNA Repair, 3. pp. 245-256. ISSN 1568-7864

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Abstract

AHNAK is a high molecular weight protein that is under-expressed in several radiosensitive neuroblastoma cell lines. Using immunoaffinity purification or purified proteins, we show that AHNAK interacts specifically with the DNA ligase IV–XRCC4 complex, a complex that functions in DNA non-homologous end-joining. Furthermore, AHNAK and the DNA ligase IV–XRCC4 complex co-immunoprecipitate demonstrating an in vivo interaction. This interaction is specific and is not observed with other DNA ligases nor with other components of the DNA non-homologous end-joining machinery. We characterised AHNAK as a protein that stimulates the double-stranded (DS) ligation activity of DNA ligase IV–XRCC4. We show that AHNAK has weak DNA-binding activity and forms a stable complex with the DNA ligase IV–XRCC4 complex on DNA. AHNAK is also able to link two DNA molecules to a similar extent to that previously reported for Ku. Together, these findings demonstrate new activities for AHNAK, and raise the possibility that it may function to modulate DNA non-homologous end-joining.

Item Type: Article
Additional Information: GDSC77
Depositing User: Gee Wheatley
Date Deposited: 20 Mar 2007
Last Modified: 10 Sep 2019 10:04
URI: http://sro.sussex.ac.uk/id/eprint/970
Google Scholar:12 Citations
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