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Replication dynamics of recombination-dependent replication forks

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Version 2 2023-06-12, 09:41
Version 1 2023-06-09, 22:49
journal contribution
posted on 2023-06-12, 09:41 authored by Karel Naiman, Eduard Campillo-Funollet, Adam WatsonAdam Watson, Alice Budden, Izumi Miyabe, Antony CarrAntony Carr
Replication forks restarted by homologous recombination are error prone and replicate both strands semi-conservatively using Pol d. Here, we use polymerase usage sequencing to visualize in vivo replication dynamics of HR-restarted forks at an S. pombe replication barrier, RTS1, and model replication by Monte Carlo simulation. We show that HR-restarted forks synthesise both strands with Pol d for up to 30?kb without maturing to a d/e configuration and that Pol a is not used significantly on either strand, suggesting the lagging strand template remains as a gap that is filled in by Pol d later. We further demonstrate that HR-restarted forks progress uninterrupted through a fork barrier that arrests canonical forks. Finally, by manipulating lagging strand resection during HR-restart by deleting pku70, we show that the leading strand initiates replication at the same position, signifying the stability of the 3' single strand in the context of increased resection.

Funding

Replication arrest, restart and genome instability; G1829; WELLCOME TRUST; 110047/Z/15/Z

History

Publication status

  • Published

File Version

  • Published version

Journal

Nature Communications

ISSN

2041-1723

Publisher

Nature Research

Volume

12

Page range

1-11

Article number

a923

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2021-01-19

First Open Access (FOA) Date

2021-02-16

First Compliant Deposit (FCD) Date

2021-01-18

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