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Optogenetic tuning reveals rho amplification-dependent dynamics of a cell contraction signal network

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posted on 2023-06-09, 22:22 authored by Dominic Kamps, Johannes Koch, Victor O Juma, Eduard Campillo-Funollet, Melanie Graessl, Soumya Banerjee, Tomáš Mazel, Xi Chen, Yao-Wen Wu, Stephanie Portet, Anotida MadzvamuseAnotida Madzvamuse, Perihan Nalbant, Leif Dehmelt
Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system of ordinary differential equations describing temporal signal network dynamics. Bifurcation analysis and numerical simulations predict a strong dependence of oscillatory system dynamics on the concentration of GEF-H1, an Lbc-type RhoGEF, which mediates the positive feedback amplification of Rho activity. This prediction is confirmed experimentally via optogenetic tuning of the effective GEF-H1 concentration in individual living cells. Numerical simulations show that pulse amplitude is most sensitive to external inputs into the myosin component at low GEF-H1 concentrations and that the spatial pulse width is dependent on GEF-H1 diffusion. Our study offers a theoretical framework to explain the emergence of local cell contraction pulses and their modulation by biochemical and mechanical signals.

History

Publication status

  • Published

File Version

  • Published version

Journal

Cell Rep

ISSN

2211-1247

Publisher

Cell Press

Issue

9

Volume

33

Page range

1-14

Article number

a108467

Event location

United States

Department affiliated with

  • Mathematics Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2020-12-08

First Open Access (FOA) Date

2020-12-08

First Compliant Deposit (FCD) Date

2020-12-07

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