T cell-dependent immune response in C1q-deficient mice: defective interferon γ production by antigen-specific T cells

Cutler, Antony J, Botto, Marina, Van Essen, Dominic, Rivi, Roberta, Davies, Kevin A, Gray, David and Walport, Mark J (1998) T cell-dependent immune response in C1q-deficient mice: defective interferon γ production by antigen-specific T cells. Journal of Experimental Medicine, 187 (11). pp. 1789-1797. ISSN 0022-1007

Full text not available from this repository.

Abstract

The role of the classical complement pathway in humoral immune responses was investigated in gene-targeted C1q-deficient mice (c1qa(-/-). Production of antigen-specific immunoglobulin (lg)g2a and Igg3 in primary and secondary responses to T cell-dependent antigen was significantly reduced, whereas Igm, Igg1, and Igg2b responses were similar in control and C1qa(-/-) mice. Despite abnormal humoral responses, B cells from C1qa(-/-) mice proliferated normally to a number of stimuli in vitro. Immune complex localization to follicular dendritic cells within splenic follicles was lacking in C1qa(-/-) mice. The precursor frequency of antigen-specific T cells was similar in C1qa(-/-) and wild-type mice. However, analysis of cytokine production by primed T cells in response to keyhole limpet hemocyanin revealed a significant reduction in interferon-γ production in C1qa(-/-) mice compared with control mice, whereas interleukin 4 secretion was equivalent. These data suggest that the classical pathway of complement may influence the cytokine profile of antigen-specific T lymphocytes and the subsequent immune response.

Item Type: Article
Keywords: Animals, Antibodies, Antigen-Antibody Complex, Antigens, B-Lymphocytes, Cell Division, Complement C1q, Complement Pathway, Classical, Female, Interferon-gamma, Interleukin-4, Male, Mice, Inbred C57BL, T-Lymphocytes
Schools and Departments: Brighton and Sussex Medical School > Brighton and Sussex Medical School
SWORD Depositor: Mx Elements Account
Depositing User: Mx Elements Account
Date Deposited: 01 Dec 2020 09:11
Last Modified: 01 Dec 2020 09:11
URI: http://sro.sussex.ac.uk/id/eprint/95410
📧 Request an update