HALL_Methods_Molecular_Biology_June_2020.pdf (1.14 MB)
Imaging pericytes and the regulation of cerebral blood flow
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posted on 2023-06-09, 21:34 authored by Katie Boyd, Matthew Hammond-Haley, Rozan Vroman, Catherine HallCatherine HallThe brain’s high energy requirements drive the need for close coupling of local neuronal activity to blood supply. Capillaries have been shown to dilate before arterioles in response to sensory stimulation, pointing to a key role for microvascular pericytes in mediating cerebrovascular dynamics. However, many aspects of these cells’ function remain unknown and even controversial, from their identification, to the mechanism and regulation of their contractility in physiology and disease. Investigating how pericytes regulate vascular diameter is therefore likely to be the subject of many future experiments. Here we provide protocols for three different techniques (ex vivo slice imaging, in vivo imaging, and immunohistochemistry) that are highly valuable for performing such experiments.
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Publication status
- Published
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- Accepted version
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Springer NaturePublisher URL
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Page range
89-117Pages
192.0Book title
Pericytes: methods and protocolsISBN
9781071610558Series
Methods in Molecular BiologyDepartment affiliated with
- Psychology Publications
Full text available
- Yes
Peer reviewed?
- No
Editors
Bruno M PeaultLegacy Posted Date
2020-09-11First Open Access (FOA) Date
2023-02-14First Compliant Deposit (FCD) Date
2020-09-11Usage metrics
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