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Localisation of human Y-family DNA polymerase ?: relationship to PCNA foci

journal contribution
posted on 2023-06-07, 13:52 authored by Tomoo Ogi, Patricia Kannouche, Alan LehmannAlan Lehmann
DNA polymerases of the Y-family are involved in translesion DNA synthesis past different types of DNA damage. Previous work has shown that DNA polymerases {eta} and {iota} are localised in replication factories during S phase, where they colocalise one-to-one with PCNA. Cells with factories containing these polymerases accumulate after treatment with DNA damaging agents because replication forks are stalled at sites of damage. We now show that DNA polymerase {kappa} (pol{kappa}) has a different localisation pattern. Although, like the other Y-family polymerases, it is exclusively localised in the nucleus, pol{kappa} is found in replication foci in only a small proportion of S-phase cells. It does not colocalise in those foci with proliferating cell nuclear antigen (PCNA) in the majority of cells. This reduced number of cells with pol{kappa} foci, when compared with those containing pol{eta} foci, is observed both in untreated cells and in cells treated with hydroxyurea, UV irradiation or benzo[a]pyrene. The C-terminal 97 amino acids of pol{kappa} are sufficient for this limited localisation into nuclear foci, and include a C2HC zinc finger, bipartite nuclear localisation signal and putative PCNA binding site.

History

Publication status

  • Published

Journal

Journal of Cell Science

ISSN

0021-9533

Publisher

Company of Biologists

Issue

1

Volume

118

Page range

129-136

Notes

GDSC167

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2007-03-19

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