PIIS2211124720306343.pdf (8.9 MB)
Prophase-specific perinuclear actin coordinates centrosome separation and positioning to ensure accurate chromosome segregation
journal contribution
posted on 2023-06-07, 07:26 authored by Thomas StiffThomas Stiff, Fabio Rodrigo Echegaray Iturra, Harry J Pink, Alex Herbert, Constantino Carlos Reyes-Aldasoro, Helfrid HocheggerHelfrid HocheggerCentrosome separation in late G2/ early prophase requires precise spatial coordination that is determined by a balance of forces promoting and antagonizing separation. The major effector of centrosome separation is the kinesin Eg5. However, the identity and regulation of Eg5-antagonizing forces is less well characterized. By manipulating candidate components, we find that centrosome separation is reversible and that separated centrosomes congress toward a central position underneath the flat nucleus. This positioning mechanism requires microtubule polymerization, as well as actin polymerization. We identify perinuclear actin structures that form in late G2/early prophase and interact with microtubules emanating from the centrosomes. Disrupting these structures by breaking the interactions of the linker of nucleoskeleton and cytoskeleton (LINC) complex with perinuclear actin filaments abrogates this centrosome positioning mechanism and causes an increase in subsequent chromosome segregation errors. Our results demonstrate how geometrical cues from the cell nucleus coordinate the orientation of the emanating spindle poles before nuclear envelope breakdown.
History
Publication status
- Published
File Version
- Published version
Journal
Cell ReportsISSN
2211-1247Publisher
ElsevierExternal DOI
Issue
8Volume
31Article number
a107681Event location
United StatesDepartment affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2020-07-07First Open Access (FOA) Date
2020-07-07First Compliant Deposit (FCD) Date
2020-07-07Usage metrics
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