Malpighamoeba infection compromises fluid secretion and P-glycoprotein detoxification in Malpighian tubules

Rossi, Marta, Ott, Swidbert R and Niven, Jeremy E (2020) Malpighamoeba infection compromises fluid secretion and P-glycoprotein detoxification in Malpighian tubules. Scientific Reports. ISSN 2045-2322 (Accepted)

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Abstract

Malpighian tubules, analogous to vertebrate nephrons, play a key role in insect osmoregulation and detoxification. Tubules can become infected with a protozoan, Malpighamoeba, which damages their epithelial cells, potentially compromising their function. Here we used a modified Ramsay assay to quantify the impact of Malpighamoeba infection on fluid secretion and P-glycoprotein-dependent detoxification by desert locust Malpighian tubules. Infected tubules have a greater surface area and a higher fluid secretion rate than uninfected tubules. Infection also impairs P-glycoprotein-dependent detoxification by reducing the net rhodamine extrusion per surface area. However, due to the increased surface area and fluid secretion rate, infected tubules have similar total net extrusion per tubule to uninfected tubules. Increased fluid secretion rate of infected tubules likely exposes locusts to greater water stress and increased energy costs. Coupled with reduced efficiency of P-glycoprotein detoxification per surface area, Malpighamoeba infection is likely to reduce insect survival in natural environments.

Item Type: Article
Keywords: Osmoregulation, Excretion, ABC transporter, Xenobiotic, Honeybee, Malameba
Schools and Departments: School of Life Sciences > Evolution, Behaviour and Environment
SWORD Depositor: Mx Elements Account
Depositing User: Mx Elements Account
Date Deposited: 01 Jul 2020 08:51
Last Modified: 01 Jul 2020 09:00
URI: http://sro.sussex.ac.uk/id/eprint/92215

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