S. pombe DNA translocases Rrp1 and Rrp2 have distinct roles at centromeres and telomeres that ensure genome stability

Barg-Wojas, Anna, Muraszko, Jakub, Kramarz, Karol, Schirmeisen, Kamila, Baranowska, Gabriela, Carr, Antony M and Dziadkowiec, Dorota (2020) S. pombe DNA translocases Rrp1 and Rrp2 have distinct roles at centromeres and telomeres that ensure genome stability. Journal of Cell Science. ISSN 1477-9137

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Abstract

The regulation of telomere and centromere structure and function is essential for maintaining genome integrity. Schizosaccharomyces pombe Rrp1 and Rrp2 are orthologues of Saccharomyces cerevisiae Uls1, a SWI2/SNF2 DNA translocase and SUMO-Targeted Ubiquitin Ligase. Here we show that Rrp1 or Rrp2 overproduction leads to chromosome instability and growth defects, a reduction of global histone levels and mislocalisation of centromere-specific histone Cnp1. These phenotypes depend on putative DNA translocase activities of Rrp1 and Rrp2, suggesting that Rrp1 and Rrp2 may be involved in modulating nucleosome dynamics. Furthermore, we confirm that Rrp2, but not Rrp1, acts at telomeres, reflecting a previously described interaction between Rrp2 and Top2. In conclusion, we identify roles for Rrp1 and Rrp2 in maintaining centromere function by modulating histone dynamics, contributing to the preservation of genome stability during vegetative cell growth.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Subjects: Q Science > Q Science (General)
Depositing User: Paula Amiet-West
Date Deposited: 22 Jan 2020 09:02
Last Modified: 22 Jan 2020 09:15
URI: http://sro.sussex.ac.uk/id/eprint/89469

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