elife-48779-v2.pdf (4.51 MB)
An arbitrary-spectrum spatial visual stimulator for vision research
journal contribution
posted on 2023-06-09, 19:45 authored by Katrin Franke, André Maia Chagas, Zhijian Zhao, Maxime Zimmermann, Philipp Bartel, Yongrong Qiu, Klaudia P Szatko, Thomas BadenThomas Baden, Thomas EulerVisual neuroscientists require accurate control of visual stimulation. However, few stimulator solutions simultaneously offer high spatio-temporal resolution and free control over the spectra of the light sources, because they rely on off-the-shelf technology developed for human trichromatic vision. Importantly, consumer displays fail to drive UV-shifted short wavelength-sensitive photoreceptors, which strongly contribute to visual behaviour in many animals, including mice, zebrafish and fruit flies. Moreover, many non-mammalian species feature more than three spectral photoreceptor types. Here, we present a flexible, spatial visual stimulator with up to six arbitrary spectrum chromatic channels. It combines a standard digital light processing engine with open source hard- and software that can be easily adapted to the experimentalist’s needs. We demonstrate the capability of this general visual stimulator experimentally in the in vitro mouse retinal whole-mount and the in vivo zebrafish. With this work, we intend to start a community effort of sharing and developing a common stimulator design for vision research.
Funding
Anisotropic retinal circuits for processing of colour and space in nature - Lister Institute Research Prize; G2503; LISTER INSTITUTE
Anisotropic retinal circuits for processing of colour and space in nature; G2397; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/R014817/1
Optical Electrophysiology: Establishing fluorescence voltage imaging capability at Sussex Neuroscience; G2018; MRC-MEDICAL RESEARCH COUNCIL
Philip Leverhulme Prize - Biological Sciences; G2276; LEVERHULME TRUST; PLP-2017-005
A window into the fly brain: "dual imaging" of neural circuits involved in locomotor behaviour in Drosophila; G2180; MRC-MEDICAL RESEARCH COUNCIL; MC_PC_15071
NeuroVisEco - Zebrafish vision in its natural context: from natural scenes through retinal and central processing to behaviour; G1871; EUROPEAN UNION; 677687
History
Publication status
- Published
File Version
- Published version
Journal
eLifeISSN
2050-084XPublisher
eLife Sciences PublicationsExternal DOI
Volume
8Page range
1-28Department affiliated with
- Neuroscience Publications
Research groups affiliated with
- Sussex Neuroscience Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2019-11-26First Open Access (FOA) Date
2019-11-26First Compliant Deposit (FCD) Date
2019-11-25Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC