Proteomic analysis of human placental syncytiotrophoblast microvesicles in preeclampsia

Baig, Sonia, Kothandaraman, Narasimhan, Manikandan, Jayapal, Rong, Li, EE, Kim Huey, Hill, Jeffrey, Lai, Chin Wee, Tan, Wan Yu, Yeoh, Felicia, Kale, Anita, Su, Lin Lin, Biswas, Arijit, Vasoo, Sheila and Choolani, Mahesh (2014) Proteomic analysis of human placental syncytiotrophoblast microvesicles in preeclampsia. Clinical Proteomics, 11. pp. 1-8. ISSN 1559-0275

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Abstract

Background
Placental syncytiotrophoblast microvesicles (STBM) are shed into the maternal circulation during normal pregnancy. STBM circulate in significantly increased amounts in preeclampsia (PE) and are considered to be among contributors to the exaggerated proinflammatory, procoagulant state of PE. However, protein composition of STBM in normal pregnancy and PE remains unknown. We therefore sought to determine the protein components of STBM and whether STBM protein expressions differ in preeclamptic and normal pregnancies.
Patients with PE (n = 3) and normal pregnant controls (n = 6) were recruited. STBM were prepared from placental explant culture supernatant. STBM proteins were analyzed by a combination of 1D Gel-LC-MS/MS. Protein expressions levels were quantified using spectral counts and validated by immunohistochemistry.
Results
Over 400 proteins were identified in the STBM samples. Among these, 25 proteins were found to be differentially expressed in preeclampsia compared to healthy pregnant controls, including integrins, annexins and histones.
Conclusion
STBM proteins include those that are implicated in immune response, coagulation, oxidative stress, apoptosis as well as lipid metabolism pathways. Differential protein expressions of STBM suggest their pathophysiological relevance in PE.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Research Centres and Groups: Sussex Drug Discovery Centre
Depositing User: Jeffrey Hill
Date Deposited: 08 Nov 2019 08:27
Last Modified: 19 Nov 2019 09:15
URI: http://sro.sussex.ac.uk/id/eprint/87894

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