Multiple sequence types responsible for healthcare-associated Acinetobacter baumannii dissemination in a single centre in Egypt

Al-Hassan, Leena, Zafer, Mai and El-Mahallawy, Hadir (2019) Multiple sequence types responsible for healthcare-associated Acinetobacter baumannii dissemination in a single centre in Egypt. BMC Infectious Diseases, 19 (829). ISSN 1471-2334

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Abstract

Background: Acinetobacter baumannii is an increasingly worrying organism in the healthcare setting, due to its
multidrug resistance and persistence. Prolonged hospitalisation, immunocompromised patients and excessive
antibiotic exposure all contribute to increasing the risk of A. baumannii infections, which makes cancer patients a
significant risk group. This study aims to investigate the dissemination of A. baumannii at the National Cancer
Institute (NCI) in Cairo – Egypt.

Methods: All bacterial isolates were typed using Multi-locus Sequence Typing (MLST) to characterise the
epidemiology of isolates. The intrinsic OXA-51-like, and the acquired carbapanemases OXA-23, − 24/40, − 58, NDM,
IMP, and VIM were also amplified and sequenced to genetically identify mechanisms of carbapenem resistance.

Results: MLST results show a high degree of multi-clonal dissemination, with 18 different Sequence Types (STs)
identified, including 5 novel. The majority of isolates belonged to International Clone (IC) 2, and carbapenem
resistance was detected in 93% of isolates and mediated by blaOXA-23, blaOXA-58, blaNDM-1 and blaVIM-1. We also
report the presence of a resistant ST732 (OXA-378) which has been previously identified in migratory birds.

Conclusions: Multiple highly resistant clones were identified in a Cancer hospital in Cairo. It is vital that clinicians
and healthcare workers are aware of the population of A. baumannii present in order to have appropriate treatment
and infection control practices.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Global Health and Infection
Research Centres and Groups: Wellcome Trust Brighton and Sussex Centre for Global Health Research
Subjects: Q Science > QR Microbiology > QR0075 Bacteria
Depositing User: Leena Al-Hassan
Date Deposited: 07 Oct 2019 15:59
Last Modified: 07 Oct 2019 16:00
URI: http://sro.sussex.ac.uk/id/eprint/86872

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Project NameSussex Project NumberFunderFunder Ref
Investigating infections and antibiotic resistance in AfricaRDF/3/024UNIVERSITY OF SUSSEXUnset