Deegan_et_al_2019.pdf (4.13 MB)
Pif1-Family helicases support fork convergence during DNA replication termination in eukaryotes
journal contribution
posted on 2023-06-09, 18:11 authored by Tom D Deegan, Jon BaxterJon Baxter, María Ángeles Ortiz Bazán, Joseph T P Yeeles, Karim P M LabibThe convergence of two DNA replication forks creates unique problems during DNA replication termination. In E. coli and SV40, the release of torsional strain by type II topoisomerases is critical for converging replisomes to complete DNA synthesis, but the pathways that mediate fork convergence in eukaryotes are unknown. We studied the convergence of reconstituted yeast replication forks that include all core replisome components and both type I and type II topoisomerases. We found that most converging forks stall at a very late stage, indicating a role for additional factors. We showed that the Pif1 and Rrm3 DNA helicases promote efficient fork convergence and completion of DNA synthesis, even in the absence of type II topoisomerase. Furthermore, Rrm3 and Pif1 are also important for termination of plasmid DNA replication in vivo. These findings identify a eukaryotic pathway for DNA replication termination that is distinct from previously characterized prokaryotic mechanisms.
Funding
Investigating how replication fork rotation causes chromosomal instability during S phase; G1903; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/N007344/1
History
Publication status
- Published
File Version
- Published version
Journal
Molecular CellISSN
1097-4164Publisher
ElsevierExternal DOI
Issue
2Volume
74Page range
231-244.e9Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Research groups affiliated with
- Genome Damage and Stability Centre Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2019-06-25First Open Access (FOA) Date
2019-06-25First Compliant Deposit (FCD) Date
2019-06-23Usage metrics
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