Leucine-glycine and carnosine dipeptides prevent diabetes induced by multiple low-dose streptozotocin in experimental model of adult mice

Vahdatpour, Tohid, Nokhodchi, Ali, Zakeri-Milani, Parvin, Mesgari-Abbasi, Mehran, Ahmadi-Asl, Naser and Valizadeh, Hadi (2019) Leucine-glycine and carnosine dipeptides prevent diabetes induced by multiple low-dose streptozotocin in experimental model of adult mice. Journal of Diabetes Investigation. ISSN 2040-1116

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Abstract

Aims/Introduction
Peptides are considered as quasi‐hormones and effective molecules for regulation of the cells function and metabolic disorders prevention. Di‐ and tripeptides with the ability to gastrointestinal absorption have been proposed to prevent diabetes progression.

Materials and Methods
Small peptides with different sequences of specific amino acids were synthesized based on a solid phase peptide synthesis (SPPS) protocol as well as carnosine (A) and glutathione (B) were examined for the prevention of diabetes induced by multiple low‐dose of streptozotocin (MLDS) in mice.

Results
The peptides A, Leu‐Gly (D) and Pro‐Pro (F) exhibited a preventive effects on blood glucose elevation and impairment of the signaling and performance of beta cells. The beta cells function assessed by immunofluorescence and blood glucose level in mice exposed to diabetes treated by the peptides A and D was similar to the normal mice. The peptide D prevented from body weight loss caused by diabetes induction. The use of D and A peptides dramatically prevents the incidence of disruption in beta cells signaling by maintaining the natural balance of intracellular Akt‐2 and cAMP.

Conclusions
The results proved that peptide D (Leu‐Gly) named Hannaneh inhibits the body weight loss caused by diabetes induction. The Hannaneh and carnosine dipeptides with preservation of normal beta cell signalling and anti DPP‐4 activity were prevented from increasing the blood glucose in mice at risk of diabetes. These dipeptides may be regarded as the pharmaceutical agents for the prevention of diabetes.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Subjects: R Medicine > RS Pharmacy and materia medica > RS0153 Materia medica > RS0160 Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
R Medicine > RS Pharmacy and materia medica > RS0153 Materia medica > RS0192 Pharmaceutical technology
Depositing User: Ali Nokhodchi
Date Deposited: 13 Feb 2019 10:19
Last Modified: 02 Jul 2019 13:01
URI: http://sro.sussex.ac.uk/id/eprint/81918

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