648.full.pdf (3.48 MB)
Proteomics of a fuzzy organelle: interphase chromatin
Version 2 2023-06-12, 08:55
Version 1 2023-06-09, 16:15
journal contribution
posted on 2023-06-12, 08:55 authored by Georg Kustatscher, Nadia Hégarat, Karen L H Willis, Cristina Furlan, Jimi-Carlo Bukowski-Wills, Helfrid HocheggerHelfrid Hochegger, Juri RappsilberChromatin proteins mediate replication, regulate expression and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle it defies classical organellar proteomics and cannot be described by a single and ultimate list of protein components. Instead we propose a new approach that provides a quantitative assessment of a protein’s probability to function in chromatin. We integrate chromatin composition over a range of different biochemical and biological conditions. This resulted in interphase chromatin probabilities for 7635 human proteins, including 1840 previously uncharacterized proteins. We demonstrate the power of our large-scale data-driven annotation during the analysis of CDK regulation in chromatin. Quantitative protein ontologies may provide a general alternative to list-based investigations of organelles and complement Gene Ontology.
Funding
Exploiting chemical genetics to investigate the control of microtubule dynamics by mitotic kinases; G0900; CANCER RESEARCH UK; C28206/A14499
History
Publication status
- Published
File Version
- Published version
Journal
The EMBO JournalISSN
0261-4189Publisher
Nature Publishing GroupExternal DOI
Issue
6Volume
33Page range
648-664Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Research groups affiliated with
- Genome Damage and Stability Centre Publications
Full text available
- Yes
Peer reviewed?
- No
Legacy Posted Date
2018-12-14First Open Access (FOA) Date
2018-12-14First Compliant Deposit (FCD) Date
2018-12-14Usage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC