Proteomics of a fuzzy organelle: interphase chromatin

Kustatscher, Georg, Hégarat, Nadia, Willis, Karen L H, Furlan, Cristina, Bukowski-Wills, Jimi-Carlo, Hochegger, Helfrid and Rappsilber, Juri (2014) Proteomics of a fuzzy organelle: interphase chromatin. The EMBO Journal, 33 (6). pp. 648-664. ISSN 0261-4189

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Abstract

Chromatin proteins mediate replication, regulate expression and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined.
This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle it defies classical organellar proteomics and cannot be described by a single and ultimate list of protein components. Instead we propose a new approach that provides a quantitative assessment of a protein’s probability to function in chromatin. We integrate
chromatin composition over a range of different biochemical and biological conditions. This resulted in interphase chromatin probabilities for 7635 human proteins, including
1840 previously uncharacterized proteins. We demonstrate the power of our large-scale data-driven annotation during the analysis of CDK regulation in chromatin. Quantitative protein ontologies may provide a general alternative to list-based investigations of organelles and complement Gene Ontology.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups: Genome Damage and Stability Centre
Subjects: Q Science > Q Science (General)
Depositing User: Sarah Frances
Date Deposited: 14 Dec 2018 12:32
Last Modified: 14 Dec 2018 12:32
URI: http://sro.sussex.ac.uk/id/eprint/80788

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Exploiting chemical genetics to investigate the control of microtubule dynamics by mitotic kinasesG0900CANCER RESEARCH UKC28206/A14499