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Early-life adversity selectively impairs a2-GABAA receptor expression in the mouse nucleus accumbens and influences the behavioral effects of cocaine
journal contribution
posted on 2023-06-09, 15:34 authored by Scott J Mitchell, Edward P Maguire, Linda Cunningham, Benjamin G Gunn, Matthias Linke, Ulrich Zechner, Claire I Dixon, Sarah KingSarah King, David N Stephens, Jerome D Swinny, Delia Belelli, Jeremy J LambertHaplotypes of the Gabra2 gene encoding the a2-subunit of the GABAA receptor (GABAAR) are associated with drug abuse, suggesting that a2-GABAARs may play an important role in the circuitry underlying drug misuse. The genetic association of Gabra2 haplotypes with cocaine addiction appears to be evident primarily in individuals who had experienced childhood trauma. Given this association of childhood trauma, cocaine abuse and the Gabra2 haplotypes, we have explored in a mouse model of early life adversity (ELA) whether such events influence the behavioral effects of cocaine and if, as suggested by the human studies, a2-GABAARs in the nucleus accumbens (NAc) are involved in these perturbed behaviors. In adult mice prior ELA caused a selective decrease of accumbal a2-subunit mRNA, resulting in a selective decrease in the number and size of the a2-subunit (but not the a1-subunit) immunoreactive clusters in NAc core medium spiny neurons (MSNs). Functionally, in adult MSNs ELA decreased the amplitude and frequency of GABAAR-mediated miniature inhibitory postsynaptic currents (mIPSCs), a profile similar to that of a2 “knock-out” (a2-/-) mice. Behaviourally, adult male ELA and a2-/- mice exhibited an enhanced locomotor response to acute cocaine and blunted sensitisation upon repeated cocaine administration, when compared to their appropriate controls. Collectively, these findings reveal a neurobiological mechanism which may relate to the clinical observation that early trauma increases the risk for substance abuse disorder (SAD) in individuals harbouring haplotypic variations in the Gabra2 gene.
Funding
Medical Research Council; G 1000008
Medical Research Council; G0802715
History
Publication status
- Published
File Version
- Published version
Journal
NeuropharmacologyISSN
0028-3908Publisher
ElsevierExternal DOI
Volume
141Page range
98-112Department affiliated with
- Psychology Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2018-10-19First Open Access (FOA) Date
2018-10-19First Compliant Deposit (FCD) Date
2018-10-18Usage metrics
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