Generating inner ear organoids containing putative cochlear hair cells from human pluripotent stem cells

Jeong, Minjin, O'Reilly, Molly, Kirkwood, Nerissa K, Al-Aama, Jumana, Lako, Majlinda, Kros, Corné J and Armstrong, Lyle (2018) Generating inner ear organoids containing putative cochlear hair cells from human pluripotent stem cells. Cell Death and Disease, 9 (9922). pp. 1-13. ISSN 2041-4889

[img] PDF - Published Version
Available under License Creative Commons Attribution.

Download (3MB)

Abstract

In view of the prevalence of sensorineural hearing defects in an ageing population, the development of protocols to generate cochlear hair cells and their associated sensory neurons as tools to further our understanding of inner ear development are highly desirable. We report herein a robust protocol for the generation of both vestibular and cochlear hair cells from human pluripotent stem cells which represents an advance over currently available methods that have been reported to generate vestibular hair cells only. Generating otic organoids from human pluripotent stem cells using a three-dimensional culture system, we show formation of both types of sensory hair cells bearing stereociliary bundles with active mechano-sensory ion channels. These cells share many morphological characteristics with their in vivo counterparts during embryonic development of the cochlear and vestibular organs and moreover demonstrate electrophysiological activity detected through single-cell patch clamping. Collectively these data represent an advance in our ability to generate cells of an otic lineage and will be useful for building models of the sensory regions of the cochlea and vestibule.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Research Centres and Groups: Sussex Neuroscience
Depositing User: Corne Kros
Date Deposited: 09 Oct 2018 14:11
Last Modified: 09 Oct 2018 14:11
URI: http://sro.sussex.ac.uk/id/eprint/79290

View download statistics for this item

📧 Request an update