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Rass2013_Article_ResolvingBranchedDNAIntermedia.pdf (812.75 kB)

Resolving branched DNA intermediates with structure-specific nucleases during replication in eukaryotes

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posted on 2023-06-09, 15:11 authored by Ulrich RassUlrich Rass
Genome duplication requires that replication forks track the entire length of every chromosome. When complications occur, homologous recombination-mediated repair supports replication fork movement and recovery. This leads to physical connections between the nascent sister chromatids in the form of Holliday junctions and other branched DNA intermediates. A key role in the removal of these recombination intermediates falls to structure-specific nucleases such as the Holliday junction resolvase RuvC in Escherichia coli. RuvC is also known to cut branched DNA intermediates that originate directly from blocked replication forks, targeting them for origin-independent replication restart. In eukaryotes, multiple structure-specific nucleases, including Mus81-Mms4/MUS81-EME1, Yen1/GEN1, and Slx1-Slx4/SLX1-SLX4 (FANCP) have been implicated in the resolution of branched DNA intermediates. It is becoming increasingly clear that, as a group, they reflect the dual function of RuvC in cleaving recombination intermediates and failing replication forks to assist the DNA replication process.

History

Publication status

  • Published

File Version

  • Published version

Journal

Chromosoma

ISSN

0009-5915

Publisher

Springer Verlag

Issue

6

Volume

122

Page range

499-515

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2018-09-24

First Open Access (FOA) Date

2018-09-24

First Compliant Deposit (FCD) Date

2018-09-21

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