Ölmezer, Gizem, Klein, Dominique and Rass, Ulrich (2015) DNA repair defects ascribed to pby1 are caused by disruption of Holliday junction resolvase Mus81-Mms4. DNA repair, 33. pp. 17-23. ISSN 1568-7856

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Abstract

PBY1 continues to be linked with DNA repair through functional genomics studies in yeast. Using the yeast knockout (YKO) strain collection, high-throughput genetic interaction screens have identified a large set of negative interactions between PBY1 and genes involved in genome stability. In drug sensitivity screens, the YKO collection pby1Δ strain exhibits a sensitivity profile typical for genes involved in DNA replication and repair. We show that these findings are not related to loss of Pby1. On the basis of genetic interaction profile similarity, we pinpoint disruption of Holliday junction resolvase Mus81-Mms4 as the mutation responsible for DNA repair phenotypes currently ascribed to pby1. The finding that Pby1 is not a DNA repair factor reconciles discrepancies in the data available for PBY1, and indirectly supports a role for Pby1 in mRNA metabolism. Data that has been collected using the YKO collection pby1Δ strain confirms and expands the chemical-genetic interactome of MUS81-MMS4.

Item Type:	Article
Keywords:	DNA repair, yeast knock-out library, Pby1, Mus81
Schools and Departments:	School of Life Sciences > Sussex Centre for Genome Damage and Stability
Research Centres and Groups:	Genome Damage and Stability Centre
Subjects:	Q Science Q Science > Q Science (General) > Q0179.9 Research
Depositing User:	Ulrich Rass
Date Deposited:	24 Sep 2018 13:28
Last Modified:	24 Sep 2018 13:28
URI:	http://sro.sussex.ac.uk/id/eprint/78928

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