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DNA repair defects ascribed to pby1 are caused by disruption of Holliday junction resolvase Mus81-Mms4.
journal contribution
posted on 2023-06-09, 15:11 authored by Gizem Ölmezer, Dominique Klein, Ulrich RassUlrich RassPBY1 continues to be linked with DNA repair through functional genomics studies in yeast. Using the yeast knockout (YKO) strain collection, high-throughput genetic interaction screens have identified a large set of negative interactions between PBY1 and genes involved in genome stability. In drug sensitivity screens, the YKO collection pby1? strain exhibits a sensitivity profile typical for genes involved in DNA replication and repair. We show that these findings are not related to loss of Pby1. On the basis of genetic interaction profile similarity, we pinpoint disruption of Holliday junction resolvase Mus81-Mms4 as the mutation responsible for DNA repair phenotypes currently ascribed to pby1. The finding that Pby1 is not a DNA repair factor reconciles discrepancies in the data available for PBY1, and indirectly supports a role for Pby1 in mRNA metabolism. Data that has been collected using the YKO collection pby1? strain confirms and expands the chemical-genetic interactome of MUS81-MMS4.
History
Publication status
- Published
Journal
DNA repairISSN
1568-7856Publisher
ElsevierExternal DOI
Volume
33Page range
17-23Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Research groups affiliated with
- Genome Damage and Stability Centre Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2018-09-24Usage metrics
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